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Aug 23

2.2. DNA Removal. Tubes were centrifuged at 2000rpm for 10min and so the pelleted muscle comprise absorbed with DNA removal buffer and proteinase k as well as typical phenol chloroform and ethanol precipitation method.

The standard and volume of the DNA are considered by agarose serum electrophoresis and spectrophotometrically and saved at -20C until utilized. On a standard we can remove 200-250 ng of DNA from condom that has been accustomed include the TVS examine during fundamental trimester ultrasonography. 2.3. HPV Typing HPV DNA examination is carried out as …

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